Product Details
| Alternative Name: | Methionine aminopeptidase 2, p67eIF2, Eukaryotic initiation factor 2-associated protein |
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| MW: | ~70kDa |
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| Source: | Produced in insect cells. Recombinant, full-length (aa 1-478) methionine aminopeptidase 2 with an N-terminal hemagglutinin (HA) tag (YPYDVPDYA), cloned from human cDNA. Produced in a baculovirus expression system. |
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| EC: | 3.4.11.18 |
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| UniProt ID: | P50579 |
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| Formulation: | Liquid. In 20mM HEPES, pH 7.3, containing 0.1M KCl, 1mM CoCl2, 1.5mM MgCl2, 0.02% sodium azide and 10% glycerol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by chromatography |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | MetAP2 is a metalloenzyme that catalyzes removal of the N-terminal methionine from proteins, with the physiologically relevant metal thought to be either cobalt or manganese. Since it was identified as the target of the anti-angiogenic natural product fumagilin (Prod. No. BML-CT100), it has been studied for its involvement in cancer, angiogenesis, inflammation, and rheumatoid arthritis. Because of differing substrate specificities and kinetics it is not desirable to substitute bacterial methionine aminopeptidase for mammalian MetAP2. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Mediomics under patents 5,885,820 and 5,888,796 |
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General Literature References
An inhibitor of methionine aminopeptidase type-2, PPI-2458, ameliorates the pathophysiological disease processes of rheumatoid arthritis: D.D. Lazarus, et al.; Inflamm. Res.
57, 18 (2008),
Abstract;
An orally delivered small-molecule formulation with antiangiogenic and anticancer activity: O. Benny, et al.; Nat. Biotechnol.
26, 799 (2008),
Abstract;
Highly potent inhibitors of methionine aminopeptidase-2 based on 1,2,4-triazole pharmacophore: J.P. Marino, Jr., et al.; J. Med. Chem.
50, 3777 (2007),
Abstract;
A methionine aminopeptidase-2 inhibitor, PPI-2458, for the treatment of rheumatoid arthritis: S.G. Bernier, et al.; PNAS USA
101, 10768 (2004),
Abstract;
Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese: J. Wang, et al.; Biochemistry
42, 5035 (2003),
Abstract;
Proteomics-based target identification: bengamides as a new class of methionine aminopeptidase inhibitors: H. Towbin, et al.; J. Biol. Chem.
278, 52964 (2003),
Abstract;
Full Text
Structure of human methionine aminopeptidase-2 complexed with fumagillin: S. Liu, et al.; Science
282, 1324 (1998),
Abstract;
The anti-angiogenic agent fumagillin covalently modifies a conserved active-site histidine in the Escherichia coli methionine aminopeptidase: W.T. Lowther, et al.; PNAS USA
95, 12153 (1998),
Abstract;
Full Text
Evidence that the human homologue of rat initiation factor associated protein (p67) is a methionine aminopeptidase: X. Li & Y.-H. Chang; BBRC
227, 152 (1996),
Abstract;
Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes: S.M. Arfin, et al.; PNAS USA
92, 7714 (1995),
Abstract;
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