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p300 (catalytic domain) (human), (recombinant)

 
BML-SE451-0100 100 µg 404.00 USD
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Product Specification

Alternative Name:E1A binding protein p300, Histone acetyltransferase p300
 
MW:45.1 kDa
 
Source:Produced in E. coli. Catalytic domain (aa 1284-1673) of human p300.
 
UniProt ID:Q09472
 
Formulation:Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Specific Activity:≥250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386 (Prod. No. BML-P198). CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis(2-nitrobenzoic acid)).
 
Application Notes:Can be used to study p300 kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example, [3H]-Acetyl CoA.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
 

Product Literature References

The histone acetyltransferase p300 inhibitor C646 reduces pro-inflammatory gene expression and inhibits histone deacetylases: T. Bosch, et al. ; Biochem. Pharmacol. 102, 130 (2016), Application(s): Incubation, Abstract;
A Novel Cell-Permeable, Selective, and Noncompetitive Inhibitor of KAT3 Histone Acetyltransferases from a Combined Molecular Pruning/Classical Isosterism Approach: C. Milite, et al.; J. Med. Chem. 58, 2779 (2015), Application(s): Surface Plasmon Resonance, Assay, Abstract; Full Text
Acetylation of Beclin 1 inhibits autophagosome maturation and promotes tumour growth: T. Sun, et al.; Nat. Commun. 6, 7215 (2015), Application(s): Incubation, Abstract; Full Text

General Literature References

Acetylation of p53 at lysine 373/382 by the histone deacetylase inhibitor depsipeptide induces expression of p21(Waf1/Cip1): Y. Zhao, et al.; Mol. Cell. Biol. 26, 2782 (2006), Abstract;
CBP and p300: HATs for different occasions: E. Kalkhoven; Biochem. Pharmacol. 68, 1145 (2004), Abstract;
CBP and p300: HATs for different occasions: E. Kalkhoven; Biochem. Pharmacol. 68, 1145 (2004), Abstract;
Regulation of the p300 HAT domain via a novel activation loop: P.R. Thompson, et al.; Nat. Struct. Mol. Biol. 11, 308 (2004), Abstract;
Acetylation regulates the DNA end-trimming activity of DNA polymerase beta: S. Hasan, et al.; Mol. Cell 10, 1213 (2002), Abstract;
Histone acetyltransferases: S.Y. Roth, et al.; Annu. Rev. Biochem. 70, 81 (2001), Abstract;
Acetylation of importin-alpha nuclear import factors by CBP/p300: A.J. Bannister, et al.; Curr. Biol. 10, 467 (2000), Abstract;
Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation: H. Kawasaki, et al.; Nature 393, 284 (1998), Abstract;
Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain: W. Gu & R.G. Roeder; Cell 90, 595 (1997), Abstract;

Related Products

p53 (368-386) 

HAT substrate
≥95% (HPLC) | Print as PDF
 
BML-P198-2500 2.5 mg 173.00 USD
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