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14-3-3ε (CT) polyclonal antibody

 
BML-SA475-0025 25 µl 100.00 USD
 
BML-SA475-0100 100 µl 320.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-215-022

Product Specification

Alternative Name:KCIP-1, Protein kinase C inhibitor protein 1, 14-3-3E
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 239-255 (CG239EEQNKEALQDVEDENQ255) of 14-3-3ε.
 
UniProt ID:P62258 (human)
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Rabbit, Sheep
 
Specificity:Specific for the ε isoform.
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (HRP/ECL detection, 1:3,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Blocking Peptide:For Blocking peptide see Prod. No. BML-SP475.
 
Formulation:Liquid. Neat serum. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
bml-sa475 wb
Figure: Western blot analysis of cell lysates using 14-3-3 ε, pAb (Prod. No. BML-SA475). Antibody dilution 1:3’000, blot was developed by HRP/ECL.1. HeLa S100 (cytosol)2. HeLa (whole cell)3. Jurkat 4. MDCK 5. A4316. MDF-77. rat brain 8. PC12
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bml-sa475 wb

Product Literature References

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005), Abstract; Full Text
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003), Abstract;
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002), Abstract;
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002), Abstract;
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999), Abstract;
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997), Abstract; Full Text
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996), Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995), Abstract;
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995), Abstract;
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994), Abstract;
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994), Abstract;
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993), Abstract;

Related Products

Blocking peptide for 14-3-3ε (CT) pAb (Prod. No. BML-SA475) 

≥80% | Print as PDF
 
BML-SP475-0100 100 µg 62.00 USD
Do you need bulk/larger quantities?
 

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