Product Details
| Alternative Name: | Sirtuin-1 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human SIRT1 (sirtuin 1). |
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| UniProt ID: | Q96EB6 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Specificity: | Recognizes recombinant and native SIRT1. |
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| Crossreactivity: | No cross-reactivity observed with recombinant human sirtuins SIRT2, SIRT3 or SIRT5. |
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band pf ~115 kDa by Western blot. Calculated molecular weight of the full-length protein is 82 kDa. |
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.4. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of recombinant human sirtuins, Jurkat cells and HeLa nuclear extract. The nitrocellulose was probed with anti- SIRT1 at 1:1000. The secondary antibody was coupled to alkaline phosphatase and the blot was developed with BCIP/NBT. Arrows indicate positions of molecular weight markers. Lanes: S2, S3, S5 (SIRTs 2, 3 & 5, 50 ng each); J (150,000 Jurkat cells); H (18 μg HeLa nuclear extract); S1 (SIRT1- 5 ng, 0.5 ng).
Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT1 antibody (Prod. no. BML-SA427) showing nuclear staining of hepatocytes.
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Product Literature References
Obesity depresses the anti-inflammatory HSP70 pathway, contributing to NAFLD progression: F.C. Di Nasco, et al.; Obesity
23, 120 (2015),
Abstract;
Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis: Z. Zhang, et al.; Arthritis Res. Ther.
13, R200 (2011),
Application(s): Immunofluorescence,
Abstract;
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