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Mca-RPPGFSAFK(Dnp)

Protease substrate
 
BML-P227-0001 1 mg 156.00 USD
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Fluorogenic substrate for ECE-1 and ECE-2, ACE1 and ACE2, neprilysin, MMP-2 and MMP-9, thimet oligopeptidase, as well as cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP-1.Mca fluorescence is quenched by the Dnp group until cleavage separates them. Ex.: 328nm, Em.: 393nm, although the following Ex/Em have also been used: 320-340/400-420. This substrate is useful for inhibitor screening and kinetic analysis.

Product Specification

Sequence:Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH[Mca=(7-methoxycoumarin-4-yl)acetyl;Dnp=2,4-dinitrophenyl]
 
MW:1388.5
 
Source:Synthetic.
 
Formulation:Lyophilized.
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white solid.
 
Solubility:Soluble in DMSO to at least 10mM.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
 
Handling:Protect from light and moisture.
 

Product Literature References

Beneficial Effects of Combined AT1 Receptor/Neprilysin Inhibition (ARNI) Versus AT1 Receptor Blockade Alone in the Diabetic Eye: T. Prasad, et al.; Invest. Ophthalmol. Vis. Sci. 57, 6722 (2016), Abstract; Full Text
Phosphinic tripeptides as dual angiotensin-converting enzyme C-domain and endothelin-converting enzyme-1 inhibitors: N. Jullien, et al.; J. Med. Chem. 53, 208 (2010), Abstract;
Flexibility in substrate recognition by thimet oligopeptidase as revealed by denaturation studies: J.A. Sigman, et al.; Biochem. J. 388, 255 (2005), Abstract; Full Text
Characterization of endothelin-converting enzyme-2. Implication for a role in the nonclassical processing of regulatory peptides: N. Mzhavia, et al.; J. Biol. Chem. 278, 14704 (2003), Abstract; Full Text
Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1: G.D. Johnson & K. Ahn; Anal. Biochem. 286, 112 (2000), Abstract;

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