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Ac-IEPD-pNA

Caspase substrate
 
BML-P133-0005 5 mg 142.00 USD
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Replaces Prod. #: ALX-260-152

Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a Km for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm.

Product Specification

Alternative Name:Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic)
 
Sequence:N-acetyl-Ile-Glu-Pro-Asp-pNA (p-nitroanilide)
 
Formula:C28H38N6O11
 
MW:634.7
 
Purity:≥95%
 
Appearance:Lyophilized solid.
 
Solubility:Soluble to at least 100mM in DMSO.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stock solution in DMSO (e.g. 0.1M) is highly stable at -20°C or -70°C.
 
Handling:Keep cool and dry.
 

Product Literature References

Definition and redesign of the extended substrate specificity of granzyme B: J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998), Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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