Ac-IEPD-pNA

Caspase substrate



BML-P133-0005	5 mg	117.00 USD

Replaces Prod. #: ALX-260-152

Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a K_m for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm.

Product Specification

Alternative Name:	Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic)

Formula:	C₂₈H₃₈N₆O₁₁

MW:	634.7

Purity:	95%

Appearance:	Lyophilized solid.

Sequence:	N-acetyl-Ile-Glu-Pro-Asp-pNA (p-nitroanilide)

Solubility:	Soluble to at least 100mM in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stock solution in DMSO (e.g. 0.1M) is highly stable at -20°C or -70°C.

Handling:	Keep cool and dry.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Product Literature References

Definition and redesign of the extended substrate specificity of granzyme B: J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998), Abstract;

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;