Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

FLUOR DE LYS® deacetylase substrate

Highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases.
 
BML-KI104-0050 50 µl 162.00 USD
Do you need bulk/larger quantities?
 
FLUOR DE LYS® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The FLUOR DE LYS® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the FLUOR DE LYS® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).

Product Specification

MW:387.4
 
Quantity:2.5 µmol (50 µl)
 
Formulation:Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO).
 
Purity:≥95% (HPLC)
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 

Product Literature References

Crystal structure of an arginase-like protein from Trypanosoma brucei that evolved without a binuclear manganese cluster: Y. Hai, et al.; Biochemistry 54, 458 (2015), Application(s): Enzyme activity assays, Abstract; Full Text
Design, Synthesis, and Evaluation of Polyamine Deacetylase Inhibitors, and High-Resolution Crystal Structures of Their Complexes with Acetylpolyamine Amidohydrolase: C. Decroos, et al.; Biochemistry 45, 4692 (2015), Application(s): Inhibitory activity of acetylpolyamine amidohydrolase assay , Abstract; Full Text
Mechanism of N-acylthiourea Mediated Activation of Human Histone Deacetylase 8 (HDAC8) at Molecular and Cellular levels: R. Singh, et al.; J. Biol. Chem. 290, 6607 (2015), Application(s): HDAC8 catalyzed reaction of human neuroblastoma cells, Abstract; Full Text
Synthesis and evaluation of N⁸-acetylspermidine analogues as inhibitors of bacterial acetylpolyamine amidohydrolase: C. Decroos, et al.; Bioorg. Med. Chem. 21, 4530 (2013), Application(s): APAH expression in E.coli analyzed using assay., Abstract; Full Text
Histone deacetylase (HDAC) inhibitors targeting HDAC3 and HDAC1 ameliorate polyglutamine-elicited phenotypes in model systems of Huntington's disease: H. Jia, et al.; Neurobiol. Dis. 46, 351 (2012), Abstract;
Improved Histone Deacetylase Inhibitors as Therapeutics for the Neurodegenerative Disease Friedreich’s Ataxia: A New Synthetic Route : C. Xu, et al.; Pharmaceuticals 4, 1578 (2011), Application(s): Assay of human FRDA lymphoblastoid cells, Abstract; Full Text

General Literature References

A microplate reader-based nonisotopic histone deacetylase activity assay: B. Heltweg and M. Jung; Anal. Biochem. 302, 175 (2002), Abstract;
A molecular mechanism of action of theophylline: Induction of histone deacetylase activity to decrease inflammatory gene expression: K. Ito et al.; PNAS 99, 8921 (2002), Abstract;
Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1: K.J. Bitterman et al.; J. Biol. Chem. 277, 45099 (2002), Abstract;
Proliferating cell nuclear antigen associates with histone deacetylase activity, integrating DNA replication and chromatin modification: S. Milutinovic et al.; J. Biol. Chem. 277, 20974 (2002), Abstract;
Cloning and characterization of a histone deacetylase, HDAC9: X. Zhou et al.; PNAS 98, 10572 (2001), Abstract;

Related Products

FLUOR DE LYS® developer I concentrate 

An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.
Print as PDF
 
BML-KI105-0300 300 µl 153.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® HDAC fluorometric cellular activity assay kit 

Monitor HDAC activity in cell culture using cell-permeable FLUOR DE LYS® substrate
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK503-0001 96 wells 445.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Brochures
Epigenetics
Epigenetics
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,