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Cathepsin S drug discovery kit

 
BML-AK431-0001 96 wells 480.00 USD
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A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.
BML-AK431 Fig1a
Example of inhibitor data. Using the control, the slope is equal to 34.28 RFU/sec. With the inhibitor (E-64), the slope is equal to 0 RFU/sec. The % activity remaining after using the inhibitor can be calculated as follow: (0/34.28) x 100 = 0%
BML-AK431 Fig2
Kinetics of Z-VVR-AMC cleavage by Cathepsin S, 0.059mU/well; 37°C. Rates were obtained from the slope of the initial, linear portion of plots of AFU vs. time. Curve and kinetic parameters derived from a non-linear least squares fit to the Michaelis-Menten equation (Marquadt algorithm).
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BML-AK431 Fig1a BML-AK431 Fig2

Product Specification

Applications:Fluorescent detection, HTS
Activity assay
 
Application Notes:Complete system designed to screen modulators of cathepsin S activity. The assays are performed in a convenient 96-well microplate format.
 
Use/Stability:The enzyme component should be thawed as close to the time of use as is possible, and held on ice while in use. The enzyme can be frozen and thawed up to 5 times, but care should be taken to minimize both the time the enzyme spends on ice, and the number of times it is frozen and thawed. Do not hold the diluted enzyme at assay temperature (e.g 37°C) for an extended time before starting the assay.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:Cathepsin S enzyme (human) (recombinant), AMC substrate, AMC calibration standard, inhibitor, assay buffer, 1/2-volume white microplate.
 
UniProt ID:P25774
 

Product Literature References

Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation: A.T. Reddy, et al.; PLoS One 11, e0153336 (2016), Application(s): Cat S enzyme inhibition assay, Abstract; Full Text

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Cathepsin
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Cathepsin Activity kit
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