The ICCB Known Bioactives Library is an important product for use in chemical genetics and drug discovery. The library is a collection of 472 diverse biologically active compounds with defined biological activity and was developed in collaboration with the Harvard Institute of Chemistry and Cell Biology. It includes the following classes of compounds: GPCR ligands, Second messenger modulators, Nuclear receptor ligands, Actin & tubulin modulators, Kinase inhibitors, Protease inhibitors, Ion channel blockers, Gene regulation agents, Lipid biosynthesis inhibitors, many other classes. Each compound is supplied dissolved in DMSO at 100µL and is ready for assaying. The library can be used for assay development and validation, mechanism profiling, lead screening or as a reference library.
Product Details
| Applications: | HTS
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| Contents: | 472 compounds. Includes compounds that affect most cellular processes and drug target classes, including: GPCR ligands, Second messenger modulators, Nuclear receptor ligands, Actin and tubulin modulators, Kinase inhibitors, Protease inhibitors, Ion channel blockers, Gene regulation agents, Lipid biosynthesis inhibitors, Phosphodiesterase inhibitors, and many other classes. |
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| Quantity: | 100µl per well |
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| Concentration: | DMSO solutions (concentration vary by compound and are provided in the documentation) |
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| Use/Stability: | Stable for at least one year from the date of receipt when stored at -80°C. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Technical Info/Product Notes: |  |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure: S. Katsumura, et al.; Cell Metab.
34, 564 (2022),
Abstract;
Academic collaborative models fostering the translation of physiological in vitro systems from basic research into drug discovery: A. Silvestri, et al.; Drug Discov. Today
26, 1369 (2021),
Abstract;
The use of informer sets in screening: perspectives on an efficient strategy to identify new probes: P.A. Clemons, et al.; SLAS Discov.
26, 855 (2021),
Abstract;
Full Text
Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion: M. Zhou, et al.; PLoS Pathog.
16, e1009022 (2020),
Application(s): Screening on transfected CHO-DSP1 cells,
Abstract;
Full Text
Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity: R. Seki, et al.; PLoS One
15, e0237030 (2020),
Abstract;
Full Text
NF-κB Signaling Negatively Regulates Osteoblast Dedifferentiation during Zebrafish Bone Regeneration: R. Mishra, et al.; Dev. Cell
52, 167 (2020),
Application(s): Screening on Juvenile and adult zebrafish,
Abstract;
Discovery of Novel Therapeutics for Muscular Dystrophies using Zebrafish Phenotypic Screens: J.J. Widrick, et al.; J. Neuromuscul. Dis.
6, 271 (2019),
Application(s): Screening on dystrophic zebrafish embryos or larvae,
Abstract;
Full Text
Entosis and apical cell extrusion constitute a tumor-suppressive mechanism downstream of Matriptase: J. Armistead, et al.; J. Cell Bio.
219, e201905190 (2019),
Application(s): Screening on zebrafish embryos,
Abstract;
Full Text
Mechanisms of Nephrogenesis Revealed by Zebrafish Chemical Screen: Prostaglandin Signaling Modulates Nephron Progenitor Fate: B.E. Chambers, et al.; Nephron
143, 68 (2019),
Abstract;
Full Text
Prostaglandin signaling regulates renal multiciliated cell specification and maturation: A.N. Marra, et al.; PNAS
116, 8409 (2019),
Abstract;
Full Text
High-Throughput Screening of Entamoeba Identifies Compounds Which Target Both Life Cycle Stages and Which Are Effective Against Metronidazole Resistant Parasites: G.M. Ehrenkaufer, et al.; Front. Cell. Infect. Microbiol.
8, 276 (2018),
Abstract;
Full Text
Identification of antipsychotic drug fluspirilene as a potential anti-glioma stem cell drug: Y. Dong, et al.; Oncotarget
8, 111728 (2017),
Abstract;
Full Text
Medium throughput biochemical compound screening identifies novel agents for pharmacotherapy of neurofibromatosis type 1: G. Semenova, et al.; Biochimie
135, 1 (2017),
Abstract;
Full Text
A dual-targeting approach to inhibit Brucella abortus replication in human cells: D.M. Czyz, et al.; Sci. Rep.
6, 35835 (2016),
Application(s): Identification of ICCB compounds that inhibit B. abortus metabolism in axenic culture,
Abstract;
Full Text
Therapeutic strategy for targeting aggressive malignant gliomas by disrupting their energy balance: A.M. Hegazy, et al.; J. Biol. Chem.
291, 21496 (2016),
Abstract;
Full Text
A novel 3D high-content assay identifies compounds that prevent fibroblast invasion into tissue surrogates: C. Wenzel, et al.; Exp. Cell. Res.
339, 35 (2015),
Abstract;
Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment: P. Li, et al.; Nature
523, 468 (2015),
Application(s): Adult zebrafish transplantation-based chemical screen,
Abstract;
3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions: C. Wenzel, et al.; Exp. Cell Res.
323, 131 (2014),
Abstract;
High-Throughput Fluorimetric Assay for 2-Hydroxyglutarate Identifies Zaprinast as a Glutaminase Inhibitor
: A. Elhammali, et al.; Cancer Discov.
4, 828 (2014),
Abstract;
High-throughput platform for the discovery of elicitors of silent bacterial gene clusters: M.R. Seyedsayamdost; Proc. Natl. Acad. Sci.
111, 7266 (2014),
Application(s): Screening of strains of
B. thailandensis E264,
Abstract;
Full Text
Retinoic acid isomers facilitate apolipoprotein E production and lipidation in astrocytes through the retinoid X receptor/retinoic acid receptor pathway: J. Zhou, et al.; J. Biol. Chem.
289, 11282 (2014),
Application(s): Screening of immortalized apoE3 astrocytes,
Abstract;
A novel four-step system for screening angiogenesis inhibitors: Q. Zhou, et al.; Mol. Med. Rep.
8, 1734 (2013),
Application(s): Injection,
Abstract;
Full Text
High-Throughput Screening of Small Molecules Identifies Hepcidin Antagonists: E. Fung, et al.; Mol. Pharmacol.
83, 681 (2013),
Abstract;
Full Text
Chemical modulation of memory formation in larval zebrafish: M.A. Wolman, et al.; Proc. Natl. Acad. Sci.
108, 15468 (2011),
Application(s): Screening of zebrafish larvae in pharmacologic experiments,
Abstract;
Full Text
Disease allele-dependent small-molecule sensitivities in blood cells from monogenic diabetes: S.Y. Shaw et al.; Proc. Natl. Acad. Sci. USA
108, 492 (2011),
Abstract;
High-content chemical and RNAi screens for suppressors of neurotoxicity in a Huntington's disease model: J. Schulte et al.; PLoS One
6, e23841 (2011),
Abstract;
Image-based screening identifies novel roles for IkappaB kinase and glycogen synthase kinase 3 in axonal degeneration
and Glycogen Synthase Kinase 3 in Axonal Degeneration: J. Gerdts, et al.; J. Biol. Chem.
286, 28011 (2011),
Application(s): Screening for delay axon degeneration following axotomy,
Abstract;
Full Text
Chemical genetics reveals bacterial and host cell functions critical for type IV effector translocation by Legionella pneumophila: X. Charpentier et al.; PLoS Pathog.
5, e1000501 (2009),
Abstract;
Identification of AML1-ETO modulators by chemical genomics: S.M. Corsello; Blood
113, 6193 (2009),
Abstract;
General Literature References
Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein: H. Pan et al.; J. Biol. Chem.
283, 33808 (2008),
Abstract;
Scaffold composition and biological relevance of screening libraries: A. Shelat et al.; Nat. Chem. Biol.
3, 442 (2007),
Abstract;
Small molecule regulators of autophagy identified by an image-based high-throughput screen: L. Zhang et al.; Proc. Natl. Acad. Sci. USA
104, 19023 (2007),
Abstract;
