Annexin V-EGFP apoptosis detection kit



ALX-850-253-KI01	25 tests	175.00 USD

ALX-850-253-KI02	100 tests	399.00 USD

ALX-850-253-KI03	400 tests	973.00 USD

EGFP (enhanced green fluorescent protein) has emerged as an ideal fluorochrome for FACS analysis and fluorescent microscopy as it offers a more intense signal than wild-type GFP. It has Ex(max): 488nm and Em(max): 530nm. The green signal produced makes EGFP ideal for dual staining with propidium iodide (PI).

Product Specification

Kit/Set Contains:	Annexin V-EGFP, propidium iodide solution and binding buffer. An assay protocol is included.

Application:	For the detection of apoptotic cells by flow cytometry.

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	+4°C

Handling:	Protect from light.

Figure: Detection of apoptosis using Annexin V-EGFP Apoptosis Detection Kit (Prod. No. ALX-850-253). Method: Apoptosis was induced in Jurkat cells by campothecin (2µM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 min. acc. to kit instructions. Results were analyzed by flow cytometry.

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