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CD163 (human) clonal antibody (K20-T)

 
ALX-810-213-R100 100 µl 215.00 USD
 
ALX-810-213-R500 500 µl 539.00 USD
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Product Specification

Alternative Name:Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor
 
Clone:K20-T
 
Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide corresponding to an N-terminal sequence of human CD163.
 
UniProt ID:Q86VB7
 
Species reactivity:Human
 
Applications:IHC (FS), IHC (PS)
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100-1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
 
Handling:After opening, prepare aliquots and store at +4°C. Avoid freeze/thaw cycles. Centrifuge the vial before use.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
 
Protocol:IHC (paraffin sections) Protocol:
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each
2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each
3. Rinse in distilled water
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes
5. Wash in distilled water for 5 minutes
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes. Remove the staining to room temperature and let the slide to cool (in TRIS-EDTA buffer, pH 9.0) for 15 minutes. (Alternatively adjust to your own protocol, keeping the required pH)
7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 15 minutes
8. Rinse in distilled water
9. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes
10. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100 for 1 hour in the closed wet chamber
11. Wash twice 5 minutes with buffer A
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB)
13. Wash twice 5 minutes with buffer A
14. Apply the chromogen (DAB), 10 minutes
15. Wash in water for 10 minutes
16. Stain in hematoxylin for 5 minutes
17. Wash in water for 10 minutes
18. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each
19. Wash the section in 2 changes of xylene for 2 minutes each
20. Mount the slide for observation
*Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween 20, pH 9.0): TRIS 1.21 g; EDTA 0,37 g; Distilled water 1000 ml. Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.
 
810-213
Figure: Formalin-fixed and paraffin-embedded human placenta (4μm) stained with cAb to CD163 (human) (Prod. No. ALX-810-213) shows strong positive cytoplasmic immunostaining of monocytes and macrophages.Kindly performed and provided by Katarína Poliaková, MD and Lubomír Straka, MD, Ph.D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
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810-213

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