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HLA-G (human) monoclonal antibody (87G)

 
ALX-805-719-C100 100 µg 399.00 USD
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Product Specification

Clone:87G
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:HLA-B27 transgenic mice were immunized with  H-2 identical murine cells transfected with expressing genes encoding HLA-G and human β2-microglobulin.
 
UniProt ID:P17693
 
Species reactivity:Human
 
Specificity:Recognizes membrane-bound and soluble HLA-G1 and HLA-G5.
 
Crossreactivity:Does not cross-react with mouse or rat HLA-G.
 
Applications:ELISA, Flow Cytometry, IHC (FS), FUNC
 
Recommended Dilutions/Conditions:ELISA (1µg/ml)
Flow Cytometry (2µg/ml)
Immunohistochemistry (frozen sections, 10µg/ml; incubate for 20 min. at 25°C)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: blocks interaction of HLA-G with inhibitory receptors.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid in PBS, pH 7.4. Sterile filtered. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

A critical look at HLA-G: R. Apps, et al.; Trends Immunol. 29, 313 (2008), Abstract;
Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition: A. Ishitani, et al.; J. Immunol. 171, 1376 (2003), Abstract; Full Text
The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy: H. Wiendl, et al.; Brain 126, 176 (2003), Abstract; Full Text
HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition: B. Riteau, et al.; Int. Immunol. 13, 193 (2001), Abstract; Full Text

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