PTX3, mAb (MNB4)



ALX-804-464-C100	100 µg	422.00 USD

Product Specification

Alternative Name:	Pentraxin 3, TSG-14, TNF stimulated gene-14

Concentration:	1mg/ml

Formulation:	Liquid. In PBS containing 0.1% sodium azide.

Clone:	MNB4

Isotype:	Rat IgG2a

Immunogen:	Recombinant human PTX3 (pentraxin 3).

Specificity:	Recognizes the N-terminus of human and rat PTX3.

Application:	ELISA (capture - excellent) Flow Cytometry Immunohistochemistry (frozen sections (1µg/ml)) Immunoprecipitation Western Blot

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Protocol:	Sandwich ELISA for PTX3 - 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 1μg/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C. - After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding. - The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer. - 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in PBS without Ca and Mg + 2% BSA were added and then incubated for 2 hours at 37°C. - The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 50ng/ml in washing buffer were added in each well. - The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer. - 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature. - After incubation the plates were washed 5 times with washing buffer and 100µl of TMB substrate solution was added to each well. After incubation for 10 min. at room-temperature, 50μl of stop-solution (1M H₂SO₄) was added. Subsequently, absorbance was read within 30min at 450nm. Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.

Figure: Dendritic cells that phagocyte Aspergillus fumigatus stained with MAb to PTX3 (MNB4) (Prod. No. ALX-804-464).

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Product Literature References

Inflammation and thrombosis in essential thrombocythemia and polycythemia vera: different role of C-reactive protein and pentraxin 3: T. Barbui, et al.; Haematologica 96, 315 (2011), Abstract; Full Text

Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages: S. Jaillon, et al.; Cell Death Differ. 16, 465 (2009), Abstract;

Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007), Abstract;

The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps: S. Jaillon, et al.; J. Exp. Med. 204, 793 (2007), Abstract;

The long Pentraxin Ptx3 is synthesized in IgA glomerulonephritis and Activates Mesangial Cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003), Abstract;

Production of the Long Pentraxin PTX3 in Advanced Atherosclerotic Plaques: M.S. Rolph, et al.; Arterioscler. Thromb. Vasc. Biol. 22, e10 (2002), Abstract;

PTX3, A Prototypical Long Pentraxin, is an Early Indicator of Acute Myocardial Infarction in Humans: G. Peri, et al.; Circulation 102, 636 (2000), Abstract; Full Text

Multimer formation and ligand recognition by the long pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997), Abstract;