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HSP90 monoclonal antibody (3G3)

 
ALX-804-079-R400 400 µl 420.00 USD
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Product Specification

Alternative Name:Heat shock protein 90
 
Clone:3G3
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Purified mouse HSP90 (heat shock protein 90).
 
UniProt ID:P07901 (HSP90α), P11499 (HSP90β)
 
Species reactivity:Human, Mouse, Rat
Chicken, Rainbow trout
 
Applications:IF, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Ammonium sulfate/ascites suspension.
 
Formulation:Liquid. Contains no preservatives.
 
Use/Stability:Before use, centrifuge suspension (4’000 x g for 15 min.) and discard supernatant. Resuspend pellet in 200µl PBS, pH 7.2. If desired, add 0.05% sodium azide. Stable for 6 months after PBS resuspension.Removal of residual ammonium sulfate is not usually necessary for IP procedure; for other uses it may be removed by dialysis or equivalent method.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Evidence that the 90-kDa heat shock protein (HSP90) exists in cytosol in heteromeric complexes containing HSP70 and three other proteins with Mr of 63,000, 56,000, and 50,000: G.H. Perdew; J. Biol. Chem. 266, 6708 (1991), Abstract; Full Text

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