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EGF receptor (human) monoclonal antibody (13G8)

 
ALX-804-064-C100 100 µg 443.00 USD
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Product Specification

Alternative Name:Epidermal growth factor receptor, EGFR, HER-1
 
Clone:13G8
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 1165-1186 of EGF receptor (epidermal growth factor receptor) conjugated to KLH.
 
UniProt ID:P00533
 
Species reactivity:Human, Mouse
 
Specificity:Recognizes the C-terminus of the EGF receptor (aa 1165-1186). Recognition is independent of phosphorylation of Tyr1173.
 
Applications:ELISA, ICC, IP, WB
Luminex
 
Recommended Dilutions/Conditions:Immunocytochemistry (0.1-1µg/ml)
Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. ALX-840-030)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Vertical suppression of the EGFR pathway prevents onset of resistance in colorectal cancers: S. Misale, et al.; Nat. Commun. 6, 8305 (2015), Application(s): Immunoblot Analysis, Abstract; Full Text
RAF Suppression Synergizes with MEK Inhibition in KRAS Mutant Cancer Cells: S. Lamba, et al.; Cell Rep. 8, 1475 (2014), Application(s): Western Blotting, Abstract; Full Text
Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer: S. Misale, et al.; Nature 486, 532 (2012), Abstract;
Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor: A. Weyergang, et al.; Biochem. Pharmacol. 74, 226 (2007), Application(s): Western blot, Abstract;

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