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Collagen I, rat tail

 
ALX-522-435-0020 20 mg 71.00 USD
 
ALX-522-435-0100 100 mg 165.00 USD
Do you need bulk/larger quantities?
 
  • New formulation enhances gelling over a larger concentration range.

Product Specification

Alternative Name:COL1
 
MW:235kDa, 215kDa, 130kDa, 115kDa
 
Source:Isolated from rat tail tendons.
 
Concentration:5mg/ml
 
Formulation:Sterile liquid. In 0.02N acetic acid.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<100 EU/mg purified protein (LAL test)
 
Appearance:Hazy viscous liquid.
 
Activity: Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips.
 
Application Notes:Can be used for preparation of collagen gels, thin layer coating of surfaces (culture plates, slides, coverslips).
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Handling:Keep sterile. Do not freeze.
 
Scientific Background:Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.
 
Protocol:Firm Gelling Procedure
(Note: The ideal concentration for gelling is 1 - 5mg/ml. The end user will need to determine the appropriate concentration for their specific purpose).


Ammonium Hydroxide Gas Method
  1. Dilute 5mg/ml collagen to the desired concentration using sterile conditions. (Note: Lower concentrations will have reduced rigidity).
  2. Add enough collagen to cover the surface.
  3. Saturate a cotton ball or piece of filter paper with concentrated ammonium hydroxide and make a closed vapor chamber.
  4. Heat to 37°C until gel forms.
  5. Remove the ammonium hydroxide.
  6. Soak the gel surface in PBS for 1 hour.
  7. Wash several times PBS.
  8. Store the gel in PBS at +4°C until use.
  9. Equilibrate the gel in the desired media for 30 minutes prior to use.

NaOH Method (For 10ml)
  1. Sterilely add 1- 7ml of 5mg/ml collagen to a tube depending on desired final concentration. (Note: Lower concentrations will have reduced rigidity).
  2. Add 1ml of sterile 10X PBS.
  3. Add 1 ml of sterile 1N NaOH.
  4. Bring final volume to 10ml with sterile dH2O
  5. Cover the desired surface with a layer of the collagen and place at 37°C until the gel has solidified. Gel will only form if the pH is ≥7.0.
  6. Store in PBS at +4°C until use.
  7. Equilibrate the gel in the desired media for 30 minutes prior to use.
 
Collagen I, rat tail CHO
Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.
Collagen I, rat tail SDS-PAGE
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, rat tail.
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Collagen I, rat tail CHO Collagen I, rat tail SDS-PAGE

Product Literature References

A filter-free blood-brain barrier model to quantitatively study transendothelial delivery of nanoparticles by fluorescence spectroscopy: E. De Jong, et al.; J. Control. Release 289, 14 (2018), Abstract;
Effects of selexipag and its active metabolite in contrasting the profibrotic myofibroblast activity in cultured scleroderma skin fibroblasts: M. Cutolo, et al.; Arthritis Res. Ther. 20, 77 (2018), Abstract; Full Text

General Literature References

Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tube: M.B. Bornstein, et al.; Lab. Invest. 134, (1958),

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