Omentin 1 (human), (recombinant)



ALX-522-108-C010	10 µg	340.00 USD

Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism.

Product Specification

Alternative Name:	Galactofuranose-binding lectin, Intestinal lactoferrin receptor, Intelectin-1, Endothelial lectin HL-1

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Lonza).

MW:	~40kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Source/Host:	Produced in HEK 293 cells. Human omentin 1 (intelectin-1) (aa 17-313) is fused at the N-terminus to a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.

Application:	Western Blot control (100ng, colorimetric)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been identified as a visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.

Background / Technical Information:	UniProt ID Q8WWA0: Intelectin-1 (human) FLAG is a registered trademark of Sigma-Aldrich Co.

SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1μg Omentin-1.

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Product Literature References

Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose: B.K. Tan, et al.; Diabetes 57, 801 (2008), Abstract;

General Literature References

Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab. 290, E1253 (2006), Abstract; Full Text

Human homologs of the Xenopus oocyte cortical granule lectin XL35: J.K. Lee, et al.; Glycobiology 11, 65 (2001), Abstract; Full Text

Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall: S. Tsuji, et al.; J. Biol. Chem. 276, 23456 (2001), Abstract; Full Text

Molecular cloning and functional expression of a human intestinal lactoferrin receptor: Y.A. Suzuki, et al.; Biochemistry 40, 15771 (2001), Abstract;