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Fc (human):CD40L (soluble) (mouse), (recombinant)

 
ALX-522-072-C010 10 µg 414.00 USD
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Product Specification

Alternative Name:CD154, TNFSF 5, gp39
 
MW:~58kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
 
UniProt ID:P27548
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse CD40.
 
Applications:ELISA
 
Application Notes:ELISA: binds to human CD40:COMP at 10-100 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that Fc (human):CD40L (mouse) stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer.
 
522-072
Figure: Proliferation of mouse B cells induced by Fc (human):CD40L, Soluble (mouse) (rec.) (Prod. No. ALX-522-072). Method: Splenocytes from Balb/c mice were purified on B220 magnetic beads using the MACS system. The resulting B cells were put in culture at a cell density of 70’000 cells/well in a 96-well plate. Cells were activated with the indicated concentrations of Fc (human):CD40L, Soluble (mouse) (rec.). After 36 hours, 0.5µCi of 3H-Thymidine/well was added. Cells were pulsed for 10 hours, then freeze-thawed, harvested and counted.
ALX-522-072 SDS-PAGE
SDS-PAGE analysis of Fc (human):CD40L (soluble) (mouse), (recombinant) (Prod. no. ALX-522-072): Lane 1: MW Marker, Lane 2: 1 µg.
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522-072 ALX-522-072 SDS-PAGE

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