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TNF-α (human), (recombinant) (cell culture grade)

ALX-520-002-C010 10 µg 190.00 USD
ALX-520-002-C050 50 µg 675.00 USD
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Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
Source:Produced in yeast.
MI:14: 9812
UniProt ID:P01375
Formulation:Lyophilized from 0.2μm-filtered solution containing mannitol.
Purity:≥97% (SDS-PAGE)
Endotoxin Content:<1EU/µg protein
Biological Activity:Induces cytolysis of mouse L929 cells in the presence of 1µg/ml Actinomycin D (Prod. No. BML-GR300) (ED50≤0.05ng/ml).
Specific Activity:≥2x107U/mg protein. Determined by standard cell cytotoxicity assay (N. Matthes & M.L. Neale; Lymphokines and Interferons, A Practical Approach; M.J. Clemens et al., eds.; IRL Press, p. 221).
Reconstitution:A quick spin is recommended followed by reconstitution in distilled, sterile water to a concentration of 0.1-1mg/ml. For further dilution, add a carrier protein to avoid loss of biological activity.
Long Term Storage:-20°C
Use/Stability:Lyophilized protein is stable at room temperature for a few weeks.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Literature References

Steroid-insensitive ERK1/2 activity drives CXCL8 synthesis and neutrophilia by airway smooth muscle: S. Robins, et al.; Am. J. Respir. Cell Mol. Biol. 45, 984 (2011), Abstract;
IL-17 Promotes p38 MAPK-Dependent Endothelial Activation Enhancing Neutrophil Recruitment to Sites of Inflammation: L. Roussel, et al.; J. Immunol. 184, 4531 (2010), Application(s): Treatment of primary LMVECs and HUVECs for assays on endothelium activation, Abstract; Full Text
Expression of an Uncleavable N-terminal RasGAP Fragment in Insulin-secreting Cells Increases Their Resistance toward Apoptotic Stimuli without Affecting Their Glucose-induced Insulin Secretion: J.Y. Yang, et al.; J. Biol. Chem. 280, 32835 (2005), Application(s): Death induction of βTC-tet cells, Abstract; Full Text
Presence of functional dendritic cells in patients chronically infected with hepatitis C virus: R.S. Longman, et al.; Blood 103, 1026 (2004), Application(s): Maturation of dendritic cells, Abstract; Full Text

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