A member of the ecdysteroid family. Analog of ecdysone with similar properties to muristerone A. It is a functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Results show that ponasterone A was able to induce expression of β-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity; EC50= 0.07 µM.
Product Details
| Alternative Name: | 25-Deoxy-20-hydroxyecdysone, 25-Deoxyecdysterone, 2β,3β,14α,20R,22R-Pentahydroxy-5β-cholest-7-en-6-one |
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| Formula: | C27H44O6 |
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| MW: | 464.6 |
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| Source: | Synthetic. |
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| CAS: | 13408-56-5 |
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| MI: | 14: 7588 |
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| Purity: | ≥95% (HPLC) |
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| Identity: | Identity determined by NMR. |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in acetic acid, 100% ethanol, methanol or DMSO; insoluble in water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2µmol). Gently mix to dissolve the product (5 to 10 min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at +4°C or at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Abstract;
Development of an in vitro Reporter Gene Assay for Screening the Ecdysone Receptor Agonists in Mysid Crustacean: M. Hirano, et al.; Interdisciplinary Studies on Environmental Chemistry
4, 141 (2010), (Book chapter),
Full Text
Perturbations in mitochondrial dynamics induced by human mutant PINK1 can be rescued by the mitochondrial division inhibitor mdivi-1: M. Cui, et al.; J. Biol. Chem.
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Full Text
Transcriptional regulation of hypoxia-inducible factor 1alpha by HIPK2 suggests a novel mechanism to restrain tumor growth: L. Nardinocchi, et al.; Biochim. Biophys. Acta
1793, 368 (2009),
Abstract;
Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol.
35, 79 (2003),
Abstract;
Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol.
13, 129 (2002),
Abstract;
Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS
97, 14512 (2000),
Abstract;
Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids
57, 649 (1992),
Abstract;
The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol.
17, 51 (1980),
Abstract;
Extraction of ponasterone A and ecdysterone from podocarpaceae and related plants: S. Imai et al.; Steroids
10, 557 (1967),
Abstract;
