Ac-DEVD-pNA

Caspase-3 inhibitor



ALX-260-033-M001	1 mg	28.00 USD

ALX-260-033-M005	5 mg	70.00 USD

Replaces Prod. #: BML-P412

Colorimetric substrate for caspase-3 (CPP32) (K_m=9.7µM) and related cysteine proteases. Sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp²¹⁶ for caspase-3 Cleavage is monitored colorimetrically at 405nm.

Product Specification

Alternative Name:	Caspase-3 substrate (chromogenic)

Formula:	C₂₆H₃₄N₆O₁₃

MW:	638.6

Purity:	≥97% (HPLC)

Appearance:	White to off-white powder.

Sequence:	Ac-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline)

Formulation:	Lyophilized.

Peptide Content:	70-95%

Solubility:	Soluble in DMSO or buffer, pH 7.

Long Term Storage:	-20°C

Use/Stability:	Always use fresh solutions.

Handling:	Keep cool and dry.

Background / Technical Information:	λmax of pNA is 400nm.

Product Literature References

Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;

Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997), Abstract; Full Text

Activation of the CPP32 protease in apoptosis induced by 1-beta-D- arabinofuranosylcytosine and other DNA-damaging agents: R. Datta, et al.; Blood 88, 1936 (1996), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature 371, 346 (1994), Abstract;

Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;