Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Ac-YVAD-AMC

Caspase-1 substrate
 
ALX-260-024-M001 1 mg 43.00 USD
 
ALX-260-024-M005 5 mg 123.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P401

Fluorogenic caspase-1 substrate with a Km=14µM. Ex.:340-360nm, Em.:440-460nm.Sequence is based on precursor interleukin-1β Asp116 cleavage site for caspase-1.

Product Specification

Alternative Name:Caspase-1 substrate (fluorogenic)
 
Sequence:Ac-Tyr-Val-Ala-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
 
Formula:C33H39N5O10
 
MW:665.7
 
Peptide Content:75-95%
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES).
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
 

Product Literature References

Crystal structure of the cysteine protease interleukin-1 β-converting enzyme: a (p20/p10)2 homodimer: N.P.C. Walker, et al.; Cell 78, 343 (1994), Abstract;
Interleukin-1 β converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract;
Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993), Abstract;
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract;
Molecular cloning of the interleukin-1 beta converting enzyme: D.P. Cerretti, et al.; Science 256, 97 (1992), Abstract;
IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha: A.D. Howard, et al.; J. Immunol. 147, 2964 (1991), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Caspase
Caspase-1
By biological activity:
Caspase Substrate
Caspase-1 Substrate
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,