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TRAIL (human) polyclonal antibody

ALX-210-732-R100 100 µl 325.00 USD
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Product Specification

Alternative Name:Apo-2L, TNFSF 10, CD253
Immunogen:Recombinant human soluble TRAIL (aa 95-281).
UniProt ID:P50591
GenBank ID:U57059
Gene/Protein Identifier:8743 (Entrez GeneID)
Source:Purified from serum.
Species reactivity:Human
Specificity:Recognizes soluble TRAIL.
Crossreactivity:Does not cross-react with mouse TRAIL.
Applications:Flow Cytometry, ICC, IHC (FS), WB
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
210-732 immunogen
Figure 1: Schematic structure of the immunogen.
Figure 2: Detection of recombinant human TRAIL (Prod. No. ALX-522-003 ) but not of recombinant human FasL (Prod. No. ALX-522-001). Method: Recombinant soluble TRAIL, and recombinant soluble FasL as negative control, were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and detected with PAb to TRAIL (human) (Prod. No. ALX-210-732) at a dilution of 1:500. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.
210-732 ICC
Figure 3: Immunostaining of HEK 293T cells transfected with a expression plasmid coding for human TRAIL (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells as indicated, cells were fixed with 4% formaldehyde or acetone for 5 min. at RT. Slides were blocked with IgG, and incubated for 1 hour with PAb to TRAIL (Prod. No. ALX-210-732) at a dilution of 1:100 or control rabbit IgG in PBS containing 1% BSA. After washes with PBS, samples were incubated with a biotinylated secondary antibody to rabbit IgG for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
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210-732 immunogen 210-732 210-732 ICC

Product Literature References

Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion: H. Dong, et al.; Nat. Med. 8, 793 (2002), Abstract;
Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8: C.M. Schempp, et al.; FEBS Lett. 493, 26 (2001), Abstract;

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