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FOXP3 (mouse) polyclonal antibody

 
ALX-210-436-C100 100 µg 290.00 USD
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Product Specification

Alternative Name:Forkhead Box protein P3, Scurfin
 
Host:Rabbit
 
Immunogen:Recombinant mouse FOXP3 (forkhead box protein P3).
 
UniProt ID:Q99JB6
 
Species reactivity:Mouse
 
Specificity:Recognizes both natural and recombinant FOXP3.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect: 1:5,000-1:10,000)
Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~46-49kDa by Western blot.
 
Purity Detail:Purified IgG.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
210-436
Figure: Western blot analysis of mouse FOXP3 with PAb to FOXP3 (mouse) (Prod. No. ALX-210-436) at 1:5’000 dilution. 1. Recombinant mouse FOXP3 protein (His tagged) 2. Transfected mouse FOXP3 full length cell lysate (HEK 293) 3. Transfected vector only cell lysate
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210-436

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