Product Details
Alternative Name: | ERβ |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 512-530 (C512SSTEDSKN KESSQNLQSQ530) of C-terminal rat ERβ (estrogen receptor β). |
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UniProt ID: | Q62986 |
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Species reactivity: | Human, Mouse, Rat
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Crossreactivity: | Does not cross-react with ERα. |
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Applications: | ICC, IF, IHC (FS), IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunofluorescence (1:100-1:1,000) Immunocytochemistry (1:100-1:1,000) Immunohistochemistry (frozen sections, 1:50) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~55kDa by Western blot. |
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Purity Detail: | Epitope-affinity purified. |
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Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure 1. Immunofluorescent analysis using ER-beta polyclonal antibody (Prod. No. ALX-210-132) shows nuclear localization of ER-beta in MCF7 cells.
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Product Literature References
Generation and reproductive phenotypes of mice lacking estrogen receptor beta: J.H. Krege, et al.; PNAS
95, 15677 (1998),
Abstract;
Full Text
Transcription and translation of estrogen receptor-beta in the male reproductive tract of estrogen receptor-alpha knock-out and wild-type mice: C.S. Rosenfeld, et al.; Endocrinology
139, 2982 (1998),
Abstract;
Distribution of estrogen-beta-like immunoreactivity in rat forebrain: X. Li, et al.; Neuroendocrinology
66, 63 (1997),
Abstract;