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PDE5A polyclonal antibody

ALX-210-099-C050 50 µg 147.00 USD
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Product Specification

Alternative Name:Phosphodiesterase 5A, cGMP-specific 3’',5’'-cyclic phosphodiesterase, CGB-PDE
Immunogen:Synthetic peptide corresponding to aa 857-875 (CA857EQQEKMLINGESGQAKRN875) of human PDE5A (phosphodiesterase 5A).
UniProt ID:O76074
Species reactivity:Mouse
Recommended Dilutions/Conditions:Western Blot (1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Epitope-affinity purified.
Formulation:Liquid. In PBS containing 30% glycerol and 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
210-099 1
Figure 1: Western blot using PAb to PDE5A (Prod. No. ALX-210-099) at 1:1’000 dilution.Lane 1) 100µg of PDE5A-deficient SKN-BE2 neuroblastoma lysate. Lane 2) 100µg lysate mixed with 5ng of purified recombinant bovine PDE5A (Prod. No. ALX-201-257). Lane 3) 3ng of partially purified recombinant bovine PDE5A. Signal was developed for 5 sec. using ECL-Plus reagent (Amersham).
210-099 2
Figure 2: Western blot using PAb to PDE5A (Prod. No. ALX-210-099) at 1:1’000 dilution.Lane 1) 200µg of 15’000 x g supernatant of lysate from mouse brain. Lane 2) Mouse heart. Lane 3) Mouse lung. Lane 4) Mouse liver. Lane 5) 5ng of purified bovine PDE5.
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Product Literature References

The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus: C. Mátyás, et al.; Cardiovasc. Diabetol. 14, 145 (2015), Application(s): Immunoblot analysis, Abstract; Full Text

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