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Follistatin (human), (recombinant)

 
ALX-201-813-0020 20 µg 214.00 USD
 
ALX-201-813-0100 100 µg 762.00 USD
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  • Carrier-free

Product Specification

Alternative Name:FS, Activin-binding protein, FSH-suppressing protein
 
MW:~31.7kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 289 amino acids.
 
UniProt ID:P19883
 
Formulation:Lyophilized from 10mM Na2PO4, pH 7.5. Sterile filtered.
 
Purity:≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <0.1ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-β family members (activin, BMPs, myostatin, GDF-11 and TGF-β1). Mechanistically, follistatin works as an antagonist by complexing with TGF-β family members to prevent them from interacting with their signaling receptors.
 
ALX-201-813 Fig1
Figure 1: Human Follistatin Bioactivity Data. MPC-11 cells were cultured with 0 to 1µg/ml human Follistatin with our without 7.5ng/ml human Activin A. Cell viability was measured after 66 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of human Follistatin was 30-50ng/ml. Complete neutralization is seen by 250ng/ml.
ALX-201-813 Fig2
Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human Follistatin has a predicted MW of 31.7 kDa.
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ALX-201-813 Fig1 ALX-201-813 Fig2

Related Products

Follistatin (mouse), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 289 amino acids., ≥90% (Reducing and Non-reducing SDS-PAGE) | Print as PDF
 
ALX-201-814-0020 20 µg 230.00 USD
 
ALX-201-814-0100 100 µg 762.00 USD
Do you need bulk/larger quantities?
 

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