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LIF (mouse), (recombinant)

 
ALX-201-242-L001 1 ml 1,117.00 USD
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Product Specification

Alternative Name:Leukemia inhibitory factor
 
MW:~20kDa
 
Source:Produced in E. coli.
 
UniProt ID:P09056
 
Quantity:107U
 
Formulation:Liquid. 0.2μm-filtered solution in PBS containing 0.5% BSA. Contains no preservatives. Further dilutions should be made with medium containing 1% BSA.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by FPLC.
 
Biological Activity:Induces macrophage differentiation and proliferation of hematopoietic stem cells; maintains indifferentiated state of of embryonic stem (ES) cell cultures; inhibits proliferation of the mouse myeloid cell line M1.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Maintenance of ES Cells: 1,000U/ml are recommended to maintain indifferentiated ES cells, R1 or E14 cells.
M1Growth Inhibition Assay:50units/ml are defined as the concentration of LIF that induces 50% of differentiated cells in tissue culture medium.
 

Product Literature References

Leukemia inhibitory factor reduces body fat mass in ovariectomized mice: J.O. Jansson, et al.; Eur. J. Endocrinol. 154, 349 (2006), Abstract;
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells: C.C. Shih, et al.; Blood 95, 1957 (2000), Abstract;
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells: R.L. Williams, et al.; Nature 336, 684 (1988), Abstract;

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