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Bone morphogenetic protein 2 (human), (recombinant)

 
ALX-201-205-C010 10 µg 328.00 USD
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Replaces Prod. #: ALX-201-205/1

Product Specification

Alternative Name:BMP-2
 
MW:~25.9kDa.
 
Source:Produced in E. coli and renatured from inclusion body material.
 
UniProt ID:P12643
 
Concentration:100-500μg/ml after reconstitution. 
 
Formulation:Lyophilized.
 
Purity:≥98% (SDS-PAGE, UV, MS)
 
Endotoxin Content:<0.02EU/mg protein.
 
Biological Activity:Induces alkaline phosphatase expression at an EC50 = 14nM in C2C12 cells.
 
Reconstitution:Reconstitute with 20-100μl 50mM sodium acetate solution, pH 4.3.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:The reconstituted solution can be further diluted in sodium acetate solution and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added.
 
Handling:After reconstitution, prepare aliquots and store at -80°C.
 

Product Literature References

Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor : F. Hillger, et al.; J. Biol. Chem. 280, 14974 (2005), Abstract; Full Text

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