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Caspase-7 (human), (recombinant) (active)

 
ALX-201-061-U025 25 U 254.00 USD
 
ALX-201-061-U100 100 U 461.00 USD
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Product Specification

Alternative Name:Mch3, CMH-1
 
Source:Produced in E. coli. Contains a C-terminal His-tag.
 
EC:3.4.22.60
 
UniProt ID:P55210
 
Formulation:Lyophilized.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:~25’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
 
Application Notes:Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
 
Reconstitution:Reconstitute to 1U/µl with PBS containing 15% glycerol.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:After reconstitution, prepare aliquots and store at -80°C.
 

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