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Estrogen receptor α monoclonal antibody (C-542)

 
ADI-SRA-1010-D 50 µg 194.00 USD
 
ADI-SRA-1010-F 200 µg 409.00 USD
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Product Specification

Alternative Name:ESR
 
Clone:C-542
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor α.
 
UniProt ID:P03372
 
GenBank ID:M12674
 
Source:Purified from ascites.
 
Species reactivity:Human, Mouse, Rat
Hamster
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:500, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of 66.3kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.
 
Estrogen receptor alpha monoclonal antibody (C-542) Schematic structure
Human Estrogen Alpha Receptor domains.
Estrogen receptor alpha monoclonal antibody (C-542) Western blot
Western blot analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).
Estrogen receptor alpha monoclonal antibody (C-542) IHC
Immunohistochemistry analysis of human Breast tumor tissue stained with Estrogen receptor α monoclonal antibody (C-542), (Prod. No. ADI-SRA-1010), at 20µg/ml.
Estrogen receptor alpha monoclonal antibody (C-542) ICC
Immunocytochemistry staining of HepG2 cells (Panel A) and MCF-7 (Panel B) with Estrogen receptor α monoclonal antibody (C-542) (Prod. No. ADI-SRA-1010) at 10µg/ml.
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Estrogen receptor alpha monoclonal antibody (C-542) Schematic structure Estrogen receptor alpha monoclonal antibody (C-542) Western blot Estrogen receptor alpha monoclonal antibody (C-542) IHC Estrogen receptor alpha monoclonal antibody (C-542) ICC

Product Literature References

Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: alterations in amygdalar MeCP2, ERK1/2 and ERα: A. Betz, et al.; Neuro. Endocrinol. Lett. 34, 347 (2013), Abstract;
Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract;
Membrane estrogen receptor-α levels in MCF-7 breast cancer cells predict cAMP and proliferation responses: D. Zivadinovic, et al.; Breast Cancer Res. 7, R101 (2005), Application(s): Cell immunoseparation, Abstract;
Regulation of the membrane estrogen receptor-alpha: role of cell density, serum, cell passage number, and estradiol: C.S. Watson, et al. ; FASEB J. 16, 1917 (2002), Application(s): ICC using rat samples, Abstract;
The novel isoform of the estrogen receptor-alpha cDNA (ERalpha isoform S cDNA) in the human testis: K. Hoshi, et al. ; J. Steroid Biochem. Mol. Biol. 80, 299 (2002), Application(s): WB using human samples, Abstract;
Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex: L.P. Freedman, et al. ; J. Biol. Chem. 275, 20928 (2000), Application(s): IP, WB using human samples, Abstract;
Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion: D. Lindholm, et al. ; PNAS 96, 10905 (1999), Application(s): ICC using rat samples, Abstract;
Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action: J. Segars, et al. ; Oncogene 16, 2513 (1998), Application(s): IP, IHC using human samples, Abstract;

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