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KDEL monoclonal antibody (10C3) (DyLight™ 488 conjugate)

 
ADI-SPA-827-488-D 50 µg 215.00 USD
 
ADI-SPA-827-488-F 200 µg 462.00 USD
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Product Specification

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
 
Clone:10C3
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
 
UniProt ID:P06761
 
GenBank ID:M14050
 
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
 
Specificity:Recognizes proteins containing the KDEL sequence.
 
Applications:Flow Cytometry
 
Recommended Dilutions/Conditions:Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
 
ADI-SPA-827-488 FC
Flow cytometry analysis of 106 Jurkat cells stained using KDEL mAb (10C3), DyLight 488™.
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ADI-SPA-827-488 FC

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