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HSP40/Hdj1 monoclonal antibody (2E1)

 
ADI-SPA-450-E 100 µg 301.00 USD
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Product Specification

Alternative Name:Heat shock protein 40
 
Clone:2E1
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human Hsp40 (Hdj1).
 
UniProt ID:P25685
 
GenBank ID:D49547
 
Species reactivity:Human, Mouse, Rat
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (2-5µg/200µl from 5x106cells)
Western Blot (1-2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~40kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.
 
SPA-450 WB
Western blot analysis of Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), NIH/3T3 (6), WR19L (7), P19 (8) and PC12 (9) using HSP40 (Hdj1), mAb (2E1).
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SPA-450 WB

Product Literature References

Mitochondrial HSP70, HSP40, and HSP60 bind to the 3' untranslated region of the Murine hepatitis virus genome: J.L. Leibowitz, et al. ; Arch. Virol. 149, 93 (2004), Application(s): WB using mouse samples, Abstract;

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