β-Catenin, mAb (12F7)



ADI-KAM-ST001-E	100 µg	235.00 USD

Product Specification

Alternative Name:	CTNNB, cadherin-associated protein

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol.

Clone:	12F7

Isotype:	Mouse IgG1

Immunogen:	Recombinant β-catenin (N-terminal fragment).

Specificity:	Recognizes human, mouse and rat β-catenin. Detects bands of ~97kDa by Western blot.

Application:	Immunohistochemistry (1-5μg/ml) Immunoprecipitation (2μg/200-300μl cell extract) Western Blot (1μg/ml, ECL) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus. This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway. Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation. β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote expression of a variety of target genes, including c-myc and cyclin D1.

Western blot analysis of A431 cells (1), Hela cells (2), NIH/3T3 cells (3), C2C12 cells (4) and Rat-1 cells (5) probed with β-catenin mAb (12F7)

Immunoprecipitation of Rat-1 cells with normal mouse IgG (1) or β-catenin mAb (12F7 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with β-catenin mAb (12F7).