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Caspase-9 monoclonal antibody (5B4)

 
ADI-AAM-139-E 100 µg 291.00 USD
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Product Specification

Clone:5B4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human caspase-9 (N-terminal fragment).
 
UniProt ID:P55211
 
GenBank ID:AAC50640
 
Species reactivity:Human, Mouse, Rat
Drosophila
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~46-50kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies.  These morphological changes occur via signalling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases.  Caspases exist as inactive proenzymes in cells, and are activated through their processing into two subunits in response to apoptotic stimulation.  Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death.  Caspase-9, also known as ICE-LAP61, Mch-62 or Apaf-33 is a 45 kDa protein that is involved in one of the relatively well characterized caspase cascades.  It is triggered by Cytochrome C release from the mitochondria, which promotes the activation of caspase-9 by forming a complex with Apaf-1 in the presence of dATP.  Once activated, caspase-9 initiates a caspase cascade involving caspase-3, -6, and -7.  The active form of caspase-9 is composed of a 32-35 kDa subunit and 10 kDa subunit.  Caspase-9 is expressed in a variety of tissues. 
 
AAM-139 WB
Western blot analysis of Caspase-9 fragments expression in apoptosis induced Jurkat cells by Fas mAb, probed with Caspase-9 mAb (5B4).
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AAM-139 WB

Product Literature References

Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species: J. Cabrera, et al.; Chem. Biol. Interact. 256, 220 (2016), Application(s): Western blot, Abstract;
Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis 12, 1183 (2007), Application(s): WB using drosophila cell lysates, Abstract;
Role of Smac in human leukaemic cell apoptosis and proliferation: A.C. Newland, et al. ; Oncogene 22, 1589 (2003), Application(s): WB using human samples, Abstract;
Bax and Bak independently promote cytochrome C release from mitochondria: E. White, et al. ; J. Biol. Chem. 277, 14127 (2002), Application(s): WB using mouse samples, Abstract;
Dysfunctional apoptosome activation in ovarian cancer: implications for chemoresistance: G. Nunez, et al. ; Cancer Res. 62, 924 (2002), Application(s): WB using human samples, Abstract;
Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene: D. Moskophidis, et al. ; Mol. Cell. Biol. 21, 8575 (2001), Application(s): WB using mouse samples, Abstract;

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