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IHC wash buffer

 
ADI-950-235-1000 1000 ml Inquire for pricing
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The IHC wash buffer is a for washing and removing excess reagents during immunostaining.

Product Specification

Formulation:Liquid. 10X wash buffer.
 
Applications:IHC
 
Shipping:Ambient
 
Long Term Storage:Ambient
 
Scientific Background:Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining.In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. The IHC wash buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining.
 
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
 
Protocol:Preparation of Reagent: Dilute one part 10x IHC wash buffer with 9 parts deionized or distilled water.
Procedure: After each immunostaining step, wash slide(s) with 1x IHC wash buffer three times. Remove excess buffer from slide and proceed to the next immunostaining step.
 

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For Research Use Only. Not for use in diagnostic procedures.
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