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[pSer727]Stat3 monoclonal antibody (23G5)

ADI-905-672-100 100 µg 419.00 USD
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Product Specification

Alternative Name:Signal transducer and activator of transcription 3
Immunogen:Synthetic peptide corresponding to a portion of Stat3 phosphorylated at Ser727.
UniProt ID:P40763
Species reactivity:Human, Mouse, Rat
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~92kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Stats (Signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, Stats enter the nucleus to regulate transcription of many different genes.
905-672 WB
Western blot analysis of untreated (1), EGF stimulated (2), or pervanadate treated (3) A431 cell lysates with Stat3 (pSer727) mAb, clone 23G5.
905-672 IHC
Immunohistochemistry analysis of human lung cancer tissue with Stat3 (pSer727) mAb, clone 23G5.
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905-672 WB 905-672 IHC

Product Literature References

Early methyl donor deficiency may induce persistent brain defects by reducing Stat3 signaling targeted by miR-124: R. Kerek, et al.; Cell Death Dis. 4, e755 (2013), Application(s): Immunofluorescence, Abstract; Full Text

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