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LTB4 ELISA kit

 
ADI-901-068 5x96 wells 1,367.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-068 (1x96 wells)
  • Ultrasensitive measurement of LTB4, detecting as little as 5.63 pg/ml
  • Widely cited in numerous peer-reviewed publications
  • Broad dynamic range suitable for a large variety of samples
  • High throughput format with results in <4 hours for up to 39 samples in duplicate
  • Highly reproducible results day-after-day and lot-after-lot
  • Fully quantitative results that surpass semi-quantitaive Western blot analysis
  • Save with bulk kit package!
The LTB4 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. Assay up to 39 samples in duplicate with a broad dynamic range that is suitable for a wide variety of sample matrices. Document your results to scientists world-wide with this highly cited kit.
ADI-900-068 std curve
ADI-900-068-Kit-Structure
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ADI-900-068 std curve ADI-900-068-Kit-Structure

Product Specification

Alternative Name:Leukotriene B4
 
Sensitivity:5.63 pg/ml (range 11.7 - 3,000 pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of LTB4 in culture supernatants, plasma, saliva, and urine from any species. Cited sample types include bronchioalveolar lavage fluid, cell lysate, crevicular fluid, tissue, serum and whole blood.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
 

Product Literature References

Homocysteine modulates 5-lipoxygenase expression level via DNA methylation: J.G. Li, et al.; Aging Cell 16, 273 (2017), Abstract; Full Text
Genetic susceptibility for air pollution-induced airway inflammation in the SALIA study: A. Huls, et al.; Environ. Res. 152, 43 (2016), Application(s): Assessment of subclinical inflammation in humans, Abstract;
Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats: E. Jursza-Piotrowska & M.J. Siemieniuch; Reprod. Biol. 16, 174 (2016), Application(s): Concentrations of leukotrienes B4 in plasma, Abstract;
iPLA2β deficiency attenuates obesity and hepatic steatosis in ob/ob mice through hepatic fatty-acyl phospholipid remodeling: X. Deng, et al.; Biochim. Biophys. Acta 1861, 449 (2016), Application(s): Measurement of serum LTB4, Abstract;
Leukotriene B4 levels in sputum from asthma patients: H. Higham, et al.; ERJ Open Res. 2, 00088-2015 (2016), Application(s): Analysis in human sputum supernatants, Abstract; Full Text
Miconidin Acetate and Primin as Potent 5-Lipoxygenase Inhibitors from Brazilian Eugenia hiemalis (Myrtaceae): G.A. Zatelli, et al.; Planta Med. Lett. (2016), Application(s): Concentration of LBT4 was measured in human cells, Full Text
Adenine suppresses IgE-mediated mast cell activation: P. Silwal, et al.; Mol. Immunol. 65, 242 (2015), Application(s): ELISA -mast cells, Abstract;
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Leukotriene B4 receptor-2 contributes to chemoresistance of SK-OV-3 ovarian cancer cells through activation of signal transducer and activator of transcription-3-linked cascade: J. Park, et al. ; Biochim. Biophys. Acta 1863, 236 (2015), Application(s): Measurement of human LTB4 , Abstract;
MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage: A.J. Lee, et al.; Exp. Mol. Med. 47, e156 (2015), Application(s): ELISA using macrophage incubations, Abstract; Full Text
5-Lipoxygenase is a candidate target for therapeutic management of stem cell-like cells in acute myeloid leukemia: J. Roos, et al.; Cancer Res. 74, 5244 (2014), Application(s): EIA using mouse hematopoietic stem and progenitor cells (HSPC) supernatant, Abstract;
Clostridium difficile-induced colitis in mice is independent of leukotrienes: B.C. Trindade, et al.; Anaerobe 30, 90 (2014), Application(s): ELISA using cecal homogenates, Abstract;
Effect of a low-fat fish oil diet on proinflammatory eicosanoids and cell-cycle progression score in men undergoing radical prostatectomy: C. Gallet, et al.; Cancer Prev. Res. (Phila) 7, 97 (2014), Abstract;
Impact of wines and wine constituents on cyclooxygenase-1, cyclooxygenase-2, and 5-lipoxygenase catalytic activity: Z. Kutil, et al.; Mediators Inflamm. 2014, 178931 (2014), Application(s): ELISA using cell lysates, Abstract; Full Text
Zileuton restores memory impairments and reverses amyloid and tau pathology in aged Alzheimer's disease mice: A. Di Meco, et al.; Neuobiol. Aging 35, 2458 (2014), Application(s): ELISA using brain homogenates, Abstract;
A highly sensitive and selective method for the determination of leukotriene B4 (LTB4) in ex vivo stimulated human plasma by ultra fast liquid chromatography-tandem mass spectrometry: W. Lin, et al.; J. Chromatogr B. analyt. Technol. Biomed. Life Sci. 925, 54 (2013), Application(s): ELISA using plasma samples, Abstract;
Leukotriene B4 receptor BLT-mediated phosphorylation of NF-κB and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products: Y.H. Nam, et al.; Microbes Infect. 13, 1211 (2011), Application(s): ELISA using culture supernatants, Abstract;
Pharmacologic blockade of 5-lipoxygenase improves the amyloidotic phenotype of an Alzheimer's disease transgenic mouse model involvement of γ-secretase: J. Chu, et al.; Am. J. Pathol. 178, 1762 (2011), Application(s): ELISA using brain homogenates, Abstract; Full Text
5-Lipoxygenase gene disruption reduces amyloid-beta pathology in a mouse model of Alzheimer's disease: T. Wisniewski, et al. ; FASEB J. 22, 1169 (2008), Application(s): EIA using mouse tissue, culture supernatants & urine, Abstract;
Comparison of the anti-inflammatory activities of imidazole antimycotics in relation to molecular structure: R. Anderson, et al. ; Chem. Biol. Drug. Des. 72, 225 (2008), Application(s): EIA using human culture supernatants, Abstract;
Delta-6-desaturase (FADS2) deficiency unveils the role of ω3-and ω6-polyunsaturated fatty acids: W. Stoffel, et al. ; EMBO J. 27, 2281 (2008), Application(s): EIA using mouse culture supernatants, Abstract;
Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K.L. Lee, et al. ; J. Med. Chem. 50, 1380 (2007), Application(s): EIA using human whole blood, Abstract;
Effects of balneotherapy on serum IL-1, PGE2 and LTB4 levels in fibromyalgia patients: F. Ardic, et al.; Rheumatol. Int. 27, 441 (2007), Application(s): ELISA using serum samples, Abstract;
The adaptogens rhodiola and schizandra modify the response to immobilization stress in rabbits by suppressing the increase of phosphorylated stress-activated protein kinase, nitric oxide and cortisol: A. Panossian, et al.; Drug Target Insights 2, 39 (2007), Application(s): ELISA using serum samples, Abstract; Full Text
TLR3-dependent induction of nitric oxide synthase in RAW 264.7 macrophage-like cells via a cytosolic phospholipase A2/cyclooxygenase-2 pathway: J. Pindado, et al.; J. Immunol. 179, 4821 (2007), Application(s): ELISA using culture supernatants, Abstract; Full Text
Effect of inhaled nitric oxide on key mediators of the inflammatory response in patients with acute lung injury: B.H. Cuthbertson, et al. ; Crit. Care Med. 28, 1736 (2000), Application(s): EIA using human bronchioalveolar lavage fluid, Abstract;

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