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[pThr183/Tyr185]Jnk1/2 ELISA kit

 
ADI-900-106 96 wells 494.00 USD
Do you need bulk/larger quantities?
 
  • Sensitive - use less precious sample per test compared to Western blot
  • Higher throughput - test up to 40 samples in duplicate in just 3 hours
  • Quantitative - obtain statistically signficant results and compare data between multiple experiments
  • Ready-to-use - save time and reduce errors with pre-coated plates
The [pThr183/Tyr185]Jnk1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:c-Jun N-terminal kinase 1/2, SAP kinase 1a/c, MAP kinase 8/9, MAPK8/9
 
Sensitivity:75.8 pg/ml (range 125 - 4,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of [pThr183/Tyr185]Jnk1/2 in cell lysates of human, mouse, and rat origin.
 
Species reactivity:Human, Mouse, Rat
 
Crossreactivity:Phospho Jnk1 (100%), phospho Jnk2 (98.6%), phospho p38 (0.42%), phospho Erk2 (0.16%) and <0.01%: non-phosphorylated Erk2, non-phosphorylated Jnk1
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 4 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2
 
Scientific Background:The Stress-activated protein kinases alpha and γ (SAPKα and γ), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinase belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.
 
UniProt ID:P45983 (Jnk1), P45984 (Jnk2)
 

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