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Protein A ELISA kit

Most sensitive (9.01 pg/ml)  colorimetric ELISA for monitoring residual contamination.
 
ADI-900-057 96 wells 460.00 USD
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  • Highly sensitive measurement, detecting as little as 9.01 pg/ml (<1 ppm) of Protein A residuals in purified humanized mAb preparations
  • Ensures accurate results as it recognizes several constructs of Protein A
  • High throughput format with results in < 3 hours for up to 37 samples in duplicate
  • Cost-effective solution compared to other technologies, providing a lowercost-per-test
  • Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
The Protein A EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Ultra-sensitive quantification ( < 1 ppm ) of Protein A residuals in purified humanized monoclonal antibody preparations. This kit recognizes four different Protein A constructs.
ADI-900-057 specificity chart 1
Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration. A: Natural Protein A from S. aureus (Millipore), n=9; B: Recombinant Protein A from E. coli (Repligen), n=9; C: Recombinant Cys-Protein A from E. coli (GE), n=12; and D: Recombinant alkaline-resistant Protein A variant from E. coli (MabSelet SuRe from GE), n=12; graphical data represents statistical mean +/- 1 standard deviation.
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ADI-900-057 specificity chart 1

Product Specification

Sensitivity:9.01 pg/ml (range 15.63 - 1,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate,Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.
 
Technical Info/Product Notes:Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided.

Application Notes:
Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics
 

Product Literature References

Indirect monitoring of protein A biosynthesis in E.coli using potentiometric multisensor system: D. Kirsanov, et al.; Sensors and Actuators B: Chemical (2016), Application(s): Measurement of Protein A,

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