Protein A ELISA kit

Most sensitive (3.35 pg/ml) colorimetric ELISA for monitoring residual contamination.



ADI-900-057	96 wells	390.00 USD

Accurate - recognizes several constructs of Protein A
Sensitive - detect < 1 ppm Protein A residuals in purified humanized monoclonal antibody preparations
Fast - results in < 3 hours from up to 37 samples in duplicate
Cost-effective - low cost-per-test

The Protein A EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Ultra-sensitive quantification ( < 1 ppm ) of Protein A residuals in purified humanized monoclonal antibody preparations. This kit recognizes four different Protein A constructs.

Figure: Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration. A: Natural Protein A from S. Aureus , n=9; B: Recombinant Protein A from E. coli, n=9; C: Recombinant Cys-Protein A from E. coli, n=12; and D: Recombinant alkaline-resistant Protein A variant from E. coli, n=12; graphical data represents statistical mean +/- 1 standard deviation.

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Product Specification

Sensitivity:	3.35 pg/ml (range 15.63 - 1,000 pg/ml)

Kit/Set Contains:	Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate,Standard, TMB Substrate, Stop solution 2

Application:	For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.

Long Term Storage:	+4°C

Miscellaneous/General:	Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.