Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

aCGH Crystal Ball Reveals Clues to Colorectal Cancer Metastasis

Over 1 million people are diagnosed with colorectal cancer (CRC) worldwide each year, making it the second and third most common cancer in women and men, respectively. A majority of these patients die as a result of metastasis occurring primarily in the liver, followed by spread to other organs. It has previously been demonstrated in human breast cancer that metastases to bone and lung can be caused by (epi)genetic alterations originally taking place in the primary tumor. In a recently published study, Mekenkamp and coworkers from various Dutch University Medical Centers characterized specific clinico-pathological features and genomic aberrations of the primary tumor in CRC patients with hepatic versus extrahepatic metastases. Using array Comparative Genomic Hybridization (aCGH) with Enzo’s CGH labeling kit for oligo arrays, they determined that patients with hepatic metastases had significantly more gains at chromosome 20p11 than patients with extrahepatic metastases. Comparison of tumors with and without 20p11 gain revealed 12 genes with expression levels that were significantly increased by the occurrence of 20p11 gain, namely XRN2, NXT1, GZF1, NAPB, CSTL1, CST3, CST5, C20orf3, ACSS1, ENTPD6, PYGB, and ABHD12. Of these 12 differentially expressed genes, C20orf3 showed the strongest correlation between copy number status, RNA expression and protein level. These promising findings could ultimately lead to the establishment of new prognostic models capable of predicting hepatic metastatic spread, and the development of liver-specific anti-metastatic therapies.

Enzo’s CGH labeling kits deliver excellent DNA yields with superior dye incorporation leading to the highest specific activity of labeling available on the market. Enzo Life Sciences also has a comprehensive portfolio for epigenetic analysis tools including deacetylation and methylation detection kits, antibodies, and biochemicals, some of which are described below:

 

Share this TechNote



References:

  • Mekenkamp LJM, et al. Chromosome 20p11 gains are associated with liver-specific metastasis in patients with colorectal cancer. Gut (2013) 62: 101..

Related Products

5-Hydroxymethylcytosine DNA ELISA kit 

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-225-0001 96 wells 613.00 USD
Do you need bulk/larger quantities?
 

SCREEN-WELL® Epigenetics library 

HTS | Print as PDF
 
BML-2836-0100 1 Library 100 µl/well 1,333.00 USD
 
BML-2836-0500 1 Library 500 µl/well 4,519.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® HDAC fluorometric cellular activity assay kit 

Monitor HDAC activity in cell culture using cell-permeable FLUOR DE LYS® substrate
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK503-0001 96 wells 570.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS®-Green HDAC fluorometric activity assay kit 

Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK530-0001 96 wells Inquire for pricing
Do you need bulk/larger quantities?
 

CGH labeling kit for BAC arrays 

CGH | Print as PDF
 
ENZ-42670 20 Reactions 968.00 USD
Do you need bulk/larger quantities?
 

CYTAG® CGH Labeling kit 

Superior labeling efficiency and better dye incorporation results in less failed runs
CGH | Print as PDF
 
ENZ-42671-K010 2x10 Reactions 983.00 USD
 
ENZ-42671-K100 2x100 Reactions 7,207.00 USD
Do you need bulk/larger quantities?
 

Express DNA methylation kit 

Fastest method of complete bisulfite conversion of DNA for methylation treatment
Print as PDF
 
ENZ-45001-0050 50 tests 311.00 USD
 
ENZ-45001-0200 200 tests 841.00 USD
Do you need bulk/larger quantities?
 

Blood and tissue DNA methylation kit 

Complete bisulfite conversion from blood and tissue samples
Print as PDF
 
ENZ-45004-0050 50 tests 311.00 USD
 
ENZ-45004-0200 200 tests 841.00 USD
Do you need bulk/larger quantities?
 

BIOARRAY™ 5-hmC methylation kit 

Detect 5-hydroxymethylcytosine using a robust glucosylation method
Print as PDF
 
ENZ-45011-0050 50 tests 702.00 USD
Do you need bulk/larger quantities?
 

Recommend this page