Digoxigenin-UTP can replace UTP in reactions catalyzed by T3, T7 or SP6 RNA polymerases. The digoxigenin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer selectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes.
Product Details
Alternative Name: | Digoxigenin-11-UTP |
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Formula: | C43H65N4O22P3 |
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MW: | 1082.92 (free acid) |
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Quantity: | 25µl |
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Concentration: | 10mM |
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Formulation: | Liquid. Solution in water. |
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Extinction Coefficient: | 22,600 M-1cm-1 (220nm, pH 7) |
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Purity: | ≥93% (HPLC) |
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Appearance: | Clear, colorless liquid. |
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Applications: | ISH (in situ hybridization), Northern Blot
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Shipping: | Dry Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. |
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Regulatory Status: | RUO - Research Use Only |
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