| ENZ-KIT103-0005 | 5x24 wells | 383.00 USD |  |
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| Do you need bulk/larger quantities? | |||||
| Applications: | Colorimetric detection |
| Application Notes: | For the induction and colorimetric detection of adipogenesis. |
| Quantity: | Contains enough reagents to conduct experiments for: 5 x 24 well plates or 225 cm2 tissue culture plate surface area |
| Use/Stability: | Store Differentiation Solution, Insulin Solution, and Enhancer Solution at -20°C. All other reagents can be stored at room temp. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Contents: | 1000x Differentiation Solution (Dexamethasone and IBMX) 1000x Insulin Solution 1000x Enhancer Solution (Rosiglitazone) 1x Cell Fixative 1x Adipogenesis Dye (Oil Red O) 1x Extraction Solution |
| Scientific Background: | The ability to regulate the cell cycle and differentiation of adipocytes are key in the development and physiology of obesity. One of the most utilized models for the study of differentiation of fibroblast into adipocytes is the 3T3-L1 cell line. Differentiation of 3T3-L1 cells into adipocytes requires three primary components: insulin or insulin-like growth factor, dexamethasone (DXM), and 3-isobutyl-1-methylxanthine (IBMX). In addition, rosiglitazone, a thiazolidinedione that binds to PPARγ and sensitizes fat cells to insulin, enhances adipogenesis. During the differentiation process, 3T3-L1 cells undergo a post-confluent mitosis, which occurs 24 hours after induction with insulin, DXM, and IBMX, followed by growth arrest. After growth arrest occurs, the cells are committed to becoming adipocytes and express late markers of differentiation at day 3, after induction. Growth arrest of the cells is a requirement for terminal adipocyte differentiation. After 5-7 days post-induction, the cell morphology changes from the elongated, fibroblastic cells to rounded cells, and lipid droplets begin to accumulate. |
| Regulatory Status: | RUO - Research Use Only |
Ciglitazone |
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| PPAR ligand | ||||||
| 74772-77-3, Synthetic., ≥98% (HPLC) | Print as PDF | ||||||
| BML-GR205-0005 | 5 mg | 141.00 USD | |
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| BML-GR205-0025 | 25 mg | 591.00 USD | |
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Indomethacin |
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| COX-1 inhibitor. PPAR activator. | ||||||
| 53-86-1, ≥98% (HPLC) | Print as PDF | ||||||
| ALX-270-086-G005 | 5 g | 44.00 USD | |
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| ALX-270-086-G025 | 25 g | 127.00 USD | |
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Linoleic acid |
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| PPAR ligand | ||||||
| 60-33-3, ≥99% | Print as PDF | ||||||
| BML-FA014-1000 | 1 g | 74.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Linolenic acid (18:3, n-3) |
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| PPAR ligand | ||||||
| 463-40-1, ≥99% (GC) | Print as PDF | ||||||
| BML-FA005-1000 | 1 g | 134.00 USD | |
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PPARγ2 (mouse) polyclonal antibody |
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| ICC, IHC, WB, FUNC, Gel Supershift | Print as PDF | ||||||
| ALX-210-192-R100 | 100 µl | 772.00 USD | |
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T0070907 |
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| PPAR ligand | ||||||
| 313516-66-4, ≥98% (TLC) | Print as PDF | ||||||
| BML-GR212-0010 | 10 mg | 122.00 USD | |
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| BML-GR212-0050 | 50 mg | 471.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Troglitazone |
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| PPAR ligand | ||||||
| 97322-87-7, Synthetic., ≥98% (HPLC) | Print as PDF | ||||||
| BML-GR210-0005 | 5 mg | 141.00 USD | |
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| BML-GR210-0025 | 25 mg | 591.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
WY-14643 |
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| PPAR ligand | ||||||
| 50892-23-4, ≥97% (HPLC) | Print as PDF | ||||||
| BML-GR200-0050 | 50 mg | 122.00 USD | |
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| BML-GR200-0250 | 250 mg | 485.00 USD | |
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BADGE |
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| PPARγ inhibitor | ||||||
| 1675-54-3, ≥85% | Print as PDF | ||||||
| ALX-270-353-G025 | 25 g | 50.00 USD | |
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Carnosic acid |
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| PPARγ activator. Antioxidant. | ||||||
| 3650-09-7, Isolated from Rosmarinus officinalis., ≥95% (HPLC) | Print as PDF | ||||||
| ALX-270-264-M010 | 10 mg | 73.00 USD | |
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| ALX-270-264-M050 | 50 mg | 215.00 USD | |
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15-Deoxy-Δ12,14-prostaglandin J2 |
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| PPARγ ligand | ||||||
| 87893-55-8, ≥97% (TLC) | Print as PDF | ||||||
| BML-PG050-0001 | 1 mg | 147.00 USD | |
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| BML-PG050-0005 | 5 mg | 646.00 USD | |
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GW1929 |
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| PPARγ agonist | ||||||
| 196808-24-9, ≥97% (HPLC) | Print as PDF | ||||||
| ALX-420-029-M001 | 1 mg | 122.00 USD | |
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| ALX-420-029-M005 | 5 mg | 575.00 USD | |
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Pioglitazone |
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| PPARγ activator | ||||||
| 111025-46-8, ≥97% (HPLC) | Print as PDF | ||||||
| ALX-270-367-M001 | 1 mg | 67.00 USD | |
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| ALX-270-367-M005 | 5 mg | 161.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Rosiglitazone |
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| Insulin sensitizing agent | ||||||
| 122320-73-4, ≥97% (HPLC) | Print as PDF | ||||||
| ALX-350-125-M025 | 25 mg | 152.00 USD | |
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| ALX-350-125-M100 | 100 mg | 431.00 USD | |
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IBMX |
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| PDE inhibitor | ||||||
| 28822-58-4, ≥99% (HPLC) | Print as PDF | ||||||
| BML-PD140-0200 | 200 mg | 76.00 USD | |
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| BML-PD140-1000 | 1 g | 295.00 USD | |
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Dexamethasone |
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| Glucocorticoid receptor ligand | ||||||
| 50-02-2, ≥98% (HPLC) | Print as PDF | ||||||
| BML-EI126-0001 | 1 g | 91.00 USD | |
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| BML-EI126-0005 | 5 g | 425.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
TNF-α (human), (recombinant) (cell culture grade) |
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| 94948-59-1, Produced in yeast., ≥97% (SDS-PAGE) | Print as PDF | ||||||
| ALX-520-002-C010 | 10 µg | 235.00 USD | |
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| ALX-520-002-C050 | 50 µg | 848.00 USD | |
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IL-1β (human), (recombinant) (cell culture grade) |
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| Potent immunomodulator | ||||||
| 97599-21-8, Produced in E. coli., ≥97% (SDS-PAGE) | Print as PDF | ||||||
| ALX-520-001-C010 | 10 µg | 490.00 USD | |
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GW-9662 |
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| PPAR ligand | ||||||
| 22978-25-2, ≥98% (HPLC) | Print as PDF | ||||||
| BML-GR234-0010 | 10 mg | 111.00 USD | |
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| BML-GR234-0050 | 50 mg | 443.00 USD | |
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