Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
UniProt ID:
P18627
GenBank ID:
NM_002286
Source:
Purified from hybridoma tissue culture supernatant.
Species reactivity:
Human
Specificity:
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3.
Applications:
Flow Cytometry, ICC
Recommended Dilutions/Conditions:
Flow Cytometry (1:100) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Protein A affinity purified.
Formulation:
Liquid. In PBS containing 50mM Tris, 1% BSA and 0.02% sodium azide.
Handling:
Do not freeze. Protect from light.
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
Regulatory Status:
RUO - Research Use Only
Flow cytometry analysis of 106 HDLM-2 cells stained using LAG-3 mAb (L4-PL33) (ATTO 647N conjugate), (Prod. No. ENZ-ABS677TS), at a concentration of 10µg/ml, side-by-side comparison to LAG-3 mAb (17B4) (ATTO 647N conjugate), (Prod. No. ALX-804-806TS).
Flow cytometry analysis of 106 HDLM-2 cells stained using LAG-3 mAb (L4-PL33) (ATTO 647N conjugate), (Prod. No. ENZ-ABS677TS), at a concentration of 10µg/ml, side-by-side comparison to LAG-3 mAb (17B4) (ATTO 647N conjugate), (Prod. No. ALX-804-806TS).