Product Details
| Alternative Name: | Dickkopf-related protein-1 |
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| Sequence: | Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus. |
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| MW: | ~40kDa (SDS-PAGE) |
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| Source: | Produced in insect cells. Produced in a baculovirus expression system. |
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| UniProt ID: | O54908 |
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| Quantity: | 5µg |
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| Formulation: | Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA. |
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| Purity: | ≥80% (SDS-PAGE) |
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| Endotoxin Content: | <1.0EU/µg (LAL method) |
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| Biological Activity: | Determined measuring the ability to inhibit the induction of the LEADING LIGHT® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001). The EC50 of this inhibition is 45-55 ng/ml. |
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| Reconstitution: | Reconstitute by adding 1.5ml water to make a 3.3µg/ml solution. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -80°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored as supplied at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | Dickkopf-related protein 1 (Dkk-1), which belongs to the dickkopf family, is a secreted protein with two cysteine rich domains and is involved in embryonic development and many human diseases. The regulation of Wnt signaling and Dkk1 inhibition has implications for bone formation and homeostatis. Dkk-1 can inhibit Wnt signaling by binding to its receptors LRP5/6 and kremen1/2 to form a complex, which can prevent Wnt from binding to LRP5/6. The predicted mouse Dkk1 protein is 272 amino acid residues with an N-terminal signal peptide. It shares 86% amino acid identity to human Dkk-1 protein. |
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| Technical Info/Product Notes: | Application Note: New LEADING LIGHT Wnt Reporter Assay with Highest Sensitivity in Response to Wnt Effectors |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Simvastatin rescues memory and granule cell maturation through the Wnt/β-catenin signaling pathway in a mouse model of Alzheimer’s disease: X.K. Tong, et al.; Cell Death Dis.
13, 325 (2022),
Abstract;
General Literature References
Function and biological roles of the Dickkopf family of Wnt modulators: C. Niehrs; Oncogene
25, 7469 (2006),
Abstract;
Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling: B. Mao et al.; Nature
417, 664 (2002),
Abstract;
LDL-receptor-related protein 6 is a receptor for Dickkopf proteins: B. Mao et al.; Nature
411, 321 (2001),
Abstract;
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