- Rapid - results in less than 4 hours
- Simple and safe - Easy colorimetric assay without radioactivity or chromatography
- Sensitive - measure as little as 0.5 μg of nuclear extract or 0.5 ng of purified enzymes
- Specific - Optimized to allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine
- Reliable - reproducible results
DNMT Colorimetric Drug Discovery Kit is a simple method to detect DNA methylation activity or inhibition with results in <4 hours.
Figure 2. Demonstration of high sensitivity and specificity of the DNMT activity assay achieved by using nuclear extracts with the DNMT Colorimetric Drug Discovery Kit. Nuclear extracts were prepared from MCF-7 cells by using the EPIXTRACT® Nuclear Protein Isolation Kit (Prod. No ENZ-45016).
Figure 1. Schematic procedure of the DNMT Colorimetric Drug Discovery Kit.
Figure 3. Demonstration of high sensitivity and specificity of DNMT activity/inhibition assay achieved by using recombinant DNMT1 with the DNMT Colorimetric Drug Discovery Kit.
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Product Details
| Alternative Name: | DNMT Activity / Inhibition Assay |
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| Assay Time: | <4 hours |
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| Applications: | Colorimetric detection
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| Application Notes: | For the quantitative determination of DNMT activity/inhibition in nuclear extracts or purified enzymes |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | Wash Buffer, DNMT Assay Buffer, Adomet, DNMT Enzyme Control, Capture Ab, Detection Ab, Enhancer Solution, Developer Solution, Stop Solution, 8-well Assay Strips with frame, Adhesive Covering Film, |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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