- Ready-to-use conversion reagent is added directly to DNA
- Easy to follow instructions
- High-yield
- DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing
The Express DNA methylation kit can be used for rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis. Simply add the Express Conversion Reagent for the reaction to occur. With the addition of heat, DNA denaturation is facilitated. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
Figure: Overview of the bisulfite conversion principle. DNA template following bisulfite treatment is sequenced. Results show that methylated cytosines (position 5) remain intact while unmethylated cytosines (positions 7,11,14,15) are converted to uracil following bisufite treatment.
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Product Details
| Use/Stability: | With proper storage, good for one year upon receipt. |
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| Handling: | Avoid exposure to light. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Contents: | Express conversion reagent, Methylation binding buffer, Methylation wash buffer concentrate, Methylation elution buffer , IC column, IC collection tube, L-Desulphonation reagent |
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| Scientific Background: | Cytosine methylation is a naturally occurring base modification, in both prokaryotic and eukaryotic organisms. It involves the addition of a methyl group to the fifth carbon position of the cytosine pyrimidine ring via a methyltransferase enzyme. It has been demonstrated that aberrant DNA methylation is a widespread phenomenon in cancer and may be among the earliest changes to occur during oncogenesis. DNA methylation has also been shown to play a central role in gene imprinting, embryonic development, X-chromosome gene silencing, and cell cycle regulation.
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Circular RNA circSEPT9 Is Upregulated in Endometrial Cancer and Promotes Cell Invasion and Migration by Downregulating miR-186 through Methylation: X. Guo, et al.; Ann. Clin. Lab. Sci.
52, 399 (2022),
Abstract;
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