Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Green 496 dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name:
5-Fluorescein dUTP
Quantity:
Sufficient for approximately 98 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Formulation:
Lyophilized.
Excitation maximum:
496 nm
Emission maximum:
520 nm
Extinction Coefficient:
85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Correction Factor (260 nm):
0.32
Correction Factor (280 nm):
0.20
Purity:
≥93% (HPLC)
Purity Detail:
Purified by ion-exchange chromatography.
Appearance:
Reddish orange solid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
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Product Literature References
Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis: J.K. Eykelenboom, et al.; J. Cell Biol. 218, 1531 (2019), Application(s): Generation of FISH probes, Abstract; Full Text
Loss of p53 causes stochastic aberrant X-chromosome inactivation and female-specific neural tube defects: A.R.D. Delbridge, et al.; Cell Rep. 27, 442 (2019), Application(s): Generation of FISH probes, Abstract;
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells: H. Ikeda, et al.; Nat. Commun. 8, 1616 (2017), Application(s): Generation of FISH probes, Abstract; Full Text
ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing: X. Chen, et al.; Nat. Methods 13, 1013 (2016), Application(s): Generation of FISH probes, Abstract; Full Text
Subtyping of renal cortical neoplasms in fine needle aspiration biopsies using a decision tree based on genomic alterations detected by fluorescence in situ hybridization: B. Gowrishankar, et al.; BJU Int. 114, 881 (2014), Abstract; Full Text
Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust: W. Liu, et al.; Theor. Appl. Genet. 122, 1537 (2011), Abstract;
Frequency of aneuploidy related to age in porcine oocytes: M. Hornak, et al.; PLoS One 8, e18894 (2011), Abstract; Full Text