ELISA (1:10,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~25kDa by Western blot.
Purity Detail:
Protein A/G-affinity purified.
Formulation:
Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
Rat lipocalin-2 is also called rat NGAL, as it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL). Rat NGAL is a 25kDa α2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas.
Regulatory Status:
RUO - Research Use Only
General Literature References
Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;
Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury: J. Mishra, et al.; J. Am. Soc. Nephrol. 14, 2534 (2003), Abstract;
Ultra sensitive (1.279 pg/ml) ELISA kit, enabling reduced input sample and matrix interference, for the quantification of KIM-1, an early biomarker for kidney injury or disease.
