Product Details
| Source: | Produced in E. coli. |
| |
| UniProt ID: | P25963 |
| |
| Formulation: | Liquid. In 50mM HEPES, pH 7.5, containing 200mM sodium chloride and 1mM DTT. |
| |
| Application Notes: | Uses:
1. NF-κB pathway studies, inhibition of NF-κB p50/p65, substrate for IκB kinase (IKK).
2.Investigation of IκBα phosphorylation/ubiquitinylation/SUMOylation.
3. Ubiquitin-proteasome mediated degradation studies. |
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -80°C |
| |
| Scientific Background: | IκBa inhibits activation of the NF-κB pathway activation by associating with the major NF-κB heterodimeric complex p50/p65 and preventing its translocation from the cytoplasm into the nucleus, where it acts a transcription factor for genes involved in inflammation, autoimmune response, cell proliferation, and apoptosis. Inhibition of NF-κB pathway activation by IκBα is controlled by its sequential serine-phosphorylation, ubiquitination and proteasomal degradation. This process itself can be inhibited by SUMOylation of IκBα, providing an additional regulatory step for the activation of the NF-κB pathway. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
SDS-PAGE analysis of Prod. No. BML-UW9975: Lane 1: MW marker, Lane 2: 1µg IκBα (human), (recombinant) (untagged).
Please mouse over
Product Literature References
An electrophoretic mobility shift assay identifies a mechanistically unique inhibitor of protein sumoylation: Y.S. Kim, et al.; Chem. Biol.
20, 604 (2013),
Abstract;
Full Text
